Coding

Part:BBa_K2910002:Design

Designed by: Victoria Sajtovich   Group: iGEM19_Toronto   (2019-10-16)


IsPETase-ML Mut 1 with C-terminal Hexahistidine Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has a C terminal hexahistidine tag for affinity purification. Hydrophobicity is optimized.


Source

This part comes from feeding PDB sequence 5XJH into the algorithm, returning the amino acid sequence, and back-translating the protein into DNA sequence using EMBOSS-Backtranseq.

References